LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1607..1703) /label=attL2 /note="attL2 (c)" misc_signal complement(1718..1737) /note="T7 Promoter/priming site (c)" primer_bind 1745..1761 /note="M13 Reverse priming site" gene 1874..2683 /note="Kanamycin resistance gene" rep_origin 2804..3477 /note="pUC origin" PCR_frame 669..1607 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..1627) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..1624) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(1615..3480,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 1585..1605 /note="TEV site" source 673..1584 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:26282 IMAGE:4824054" /tissue_type="Testis" /clone_lib="NIH_MGC_97" /lab_host="DH10B" /note="Vector: pBluescriptR" gene 673..1584 /gene="GNPTG" /gene_synonym=LP2537 /db_xref="GeneID:84572" /db_xref="HGNC:23026" /db_xref="MIM:607838" CDS 673..1584 /dnas_title="N-acetylglucosamine-1-phosphate transferase, gamma subunit" /gene="GNPTG" /gene_synonym=LP2537 /codon_start=1 /product="N-acetylglucosamine-1-phosphate transferase, gamma subunit" /protein_id="AAH14592.1" /db_xref="GI:15779035" /db_xref="GeneID:84572" /db_xref="HGNC:23026" /db_xref="MIM:607838" /translation="MAAGLARLLLLLGLSAGGPAPAGAAKMKVVEEPNAFGVNNPFLP QASRLQAKRDPSPVSGPVHLFRLSGKCFSLVESTYKYEFCPFHNVTQHEQTFRWNAYS GILGIWHEWEIANNTFTGMWMRDGDACRSRSRQSKVELACGKSNRLAHVSEPSTCVYA LTFETPLVCHPHALLVYPTLPEALQRQWDQVEQDLADELITPQGHEKLLRTLFEDAGY LKTPENEPTQLEGGPDSLGFETLENCRKAHKELSKEIKRLKGLLTQHGIPYTRPTETS NLEHLGHETPRAKSPEQLRGDPGLRGSL" misc_feature 673..1584 /note="GNPTG coding region" misc_difference 1341..1342 /gene="GNPTG" /gene_synonym=LP2537 /note="3 bases in the human genome, GAA, are not found in cDNA." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatggcggc ggggctggcg cggctcctgt tgctcctcgg gctctcggcc 721 ggcgggcccg cgccggcagg tgcagcgaag atgaaggtgg tggaggagcc caacgcgttt 781 ggggtgaaca acccgttctt gcctcaggcc agtcgcctcc aggccaagag ggatccttca 841 cccgtgtctg gacccgtgca tctcttccga ctctcgggca agtgcttcag cctggtggag 901 tccacgtaca agtatgagtt ctgcccgttc cacaacgtga cccagcacga gcagaccttc 961 cgctggaacg cctacagtgg gatcctcggc atctggcacg agtgggagat cgccaacaac 1021 accttcacgg gcatgtggat gagggacggt gacgcctgcc gttcccggag ccggcagagc 1081 aaggtggagc tggcgtgtgg aaaaagcaac cggctggccc atgtgtccga gccgagcacc 1141 tgcgtctacg cgctgacgtt cgagaccccc ctcgtctgcc acccccacgc cttgctagtg 1201 tacccaaccc tgccagaggc cctgcagcgg cagtgggacc aggtagagca ggacctggcc 1261 gatgagctga tcacccccca gggccatgag aagttgctga ggacactttt tgaggatgct 1321 ggctacttaa agaccccaga aaatgaaccc acccagctgg agggaggtcc tgacagcttg 1381 gggtttgaga ccctggaaaa ctgcaggaag gctcataaag aactctcaaa ggagatcaaa 1441 aggctgaaag gtttgctcac ccagcacggc atcccctaca cgaggcccac agaaacttcc 1501 aacttggagc acttgggcca cgagacgccc agagccaagt ctccagagca gctgcggggt 1561 gacccaggac tgcgtgggag tttggaaaac ttgtactttc aaggctCCAG CTTTCTTGTA 1621 CAAAGTTGGC ATTATAAGAA AGCATTGCTT ATCAATTTGT TGCAACGAAC AGGTCACTAT 1681 CAGTCAAAAT AAAATCATTA TTTGCCATCC AGCTGATATC CCCTATAGTG AGTCGTATTA 1741 CATGGTCATA GCTGTTTCCT GGCAGCTCTG GCCCGTGTCT CAAAATCTCT GATGTTACAT 1801 TGCACAAGAT AAAATAATAT CATCATGAAC AATAAAACTG TCTGCTTACA TAAACAGTAA 1861 TACAAGGGGT GTTATGAGCC ATATTCAACG GGAAACGTCG AGGCCGCGAT TAAATTCCAA 1921 CATGGATGCT GATTTATATG GGTATAAATG GGCTCGCGAT AATGTCGGGC AATCAGGTGC 1981 GACAATCTAT CGCTTGTATG GGAAGCCCGA TGCGCCAGAG TTGTTTCTGA AACATGGCAA 2041 AGGTAGCGTT GCCAATGATG TTACAGATGA GATGGTCAGA CTAAACTGGC TGACGGAATT 2101 TATGCCTCTT CCGACCATCA AGCATTTTAT CCGTACTCCT GATGATGCAT GGTTACTCAC 2161 CACTGCGATC CCCGGAAAAA CAGCATTCCA GGTATTAGAA GAATATCCTG ATTCAGGTGA 2221 AAATATTGTT GATGCGCTGG CAGTGTTCCT GCGCCGGTTG CATTCGATTC CTGTTTGTAA 2281 TTGTCCTTTT AACAGCGATC GCGTATTTCG TCTCGCTCAG GCGCAATCAC GAATGAATAA 2341 CGGTTTGGTT GATGCGAGTG ATTTTGATGA CGAGCGTAAT GGCTGGCCTG TTGAACAAGT 2401 CTGGAAAGAA ATGCATAAAC TTTTGCCATT CTCACCGGAT TCAGTCGTCA CTCATGGTGA 2461 TTTCTCACTT GATAACCTTA TTTTTGACGA GGGGAAATTA ATAGGTTGTA TTGATGTTGG 2521 ACGAGTCGGA ATCGCAGACC GATACCAGGA TCTTGCCATC CTATGGAACT GCCTCGGTGA 2581 GTTTTCTCCT TCATTACAGA AACGGCTTTT TCAAAAATAT GGTATTGATA ATCCTGATAT 2641 GAATAAATTG CAGTTTCATT TGATGCTCGA TGAGTTTTTC TAATCAGAAT TGGTTAATTG 2701 GTTGTAACAC TGGCAGAGCA TTACGCTGAC TTGACGGGAC GGCGCAAGCT CATGACCAAA 2761 ATCCCTTAAC GTGAGTTACG CGTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA 2821 AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC 2881 ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT 2941 AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC CGTAGTTAGG 3001 CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC 3061 AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT 3121 ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA 3181 GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT 3241 TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG 3301 CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA 3361 CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA 3421 CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT //